PathPlus(tm) TYRP1 / gp75 Antibody (aa396-445)

LifeSpan BioSciences

PathPlus(tm) TYRP1 / gp75 Antibody (aa396-445)

23

Immunohistochemistry: LS-B4011 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4011 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1microg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.. IHC, IHC-P (5 µg/ml), WB (1 µg/ml) Immunohistochemistry: LS-B4011 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4011 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.5 mg/ml

Unconjugated

TYRP1

tyrosinase related protein 1

CAS2, CATB, GP75, OCA3, TRP, TRP1, TYRP, b-PROTEIN, 5,6-dihydroxyindole-2-carboxylic acid oxidase, DHICA oxidase, catalase B, glycoprotein 75, melanoma antigen gp75

Rabbit

IgG

Human, Mouse

-20°C,2°C to 8°C

41116161