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Western Blot analysis of 293T cells using Pax-1 Polyclonal Antibody
Western Blot analysis of 293T cells using Pax-1 Polyclonal Antibody
Western Blot analysis of 293T cells using Pax-1 Polyclonal Antibody

PAX1 Antibody

CSB-PA020238
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPAX1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PAX1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5075
  • Target name
    PAX1
  • Target description
    paired box 1
  • Target synonyms
    HUP48; OFC2; paired box gene 1; paired box protein Pax-1; paired domain gene HuP48
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15863
  • Protein Name
    Paired box protein Pax-1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PAX1 using anti-PAX1 antibody (A04559-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Sw579 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat ovary tissue lytsates, Lane 7: rat lung tissue lysates, Lane 8: mouse thymus tissue lysates, Lane 9: mouse ovary tissue lysates, Lane 10: mouse lung tissue lysates, Lane 11: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX1 antigen affinity purified polyclonal antibody (Catalog # A04559-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX1 at approximately 60KD. The expected band size for PAX1 is at 60KD.
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