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Western Blot analysis of 293 cells using Pax-2 Polyclonal Antibody
Western Blot analysis of 293 cells using Pax-2 Polyclonal Antibody
Western Blot analysis of 293 cells using Pax-2 Polyclonal Antibody

PAX2 Antibody

CSB-PA003709
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPAX2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PAX2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5076
  • Target name
    PAX2
  • Target description
    paired box 2
  • Target synonyms
    FSGS7; paired box homeotic gene 2; paired box protein Pax-2; PAPRS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02962
  • Protein Name
    Paired box protein Pax-2
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Pax2 using anti-Pax2 antibody (PB9734). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Lung Tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pax2 antigen affinity purified polyclonal antibody (Catalog # PB9734) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pax2 at approximately 45 kDa. The expected band size for Pax2 is at 45 kDa.
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