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Paxillin antibody detects Paxillin protein at cytoskeleton by immunofluorescent analysis. Sample: MDA-MB-231 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Paxillin protein stained by Paxillin antibody (GTX125891) diluted at 1:100. Red: phalloidin staining. Courtesy of a researcher who prefers to be anonymous.
Paxillin antibody detects Paxillin protein at cytoskeleton by immunofluorescent analysis. Sample: MDA-MB-231 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Paxillin protein stained by Paxillin antibody (GTX125891) diluted at 1:100. Red: phalloidin staining. Courtesy of a researcher who prefers to be anonymous.
Paxillin antibody detects Paxillin protein at cytoskeleton by immunofluorescent analysis. Sample: MDA-MB-231 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Paxillin protein stained by Paxillin antibody (GTX125891) diluted at 1:100. Red: phalloidin staining. Courtesy of a researcher who prefers to be anonymous.

Paxillin antibody

GTX125891
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPXN
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Overview

  • Supplier
    GeneTex
  • Product Name
    Paxillin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.08 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5829
  • Target name
    PXN
  • Target description
    paxillin
  • Target synonyms
    paxillin, testicular tissue protein Li 134
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49023
  • Protein Name
    Paxillin
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Paxillin/PXN using anti-Paxillin/PXN antibody (A01033-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Paxillin/PXN antigen affinity purified polyclonal antibody (Catalog # A01033-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Paxillin/PXN at approximately 65 kDa. The expected band size for Paxillin/PXN is at 65 kDa.
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