![Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr31) antibody [HL3129] (GTX640615) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr31) antibody [HL3129] (GTX640615) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640615/GTX640615_T-45467_20240719_WB_M_treament_Pervanadate_24072519_165.webp)
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr31) antibody [HL3129] (GTX640615) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Paxillin (phospho Tyr31) antibody [HL3129]
GTX640615
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPXN
Overview
- SupplierGeneTex
- Product NamePaxillin (phospho Tyr31) antibody [HL3129]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3129
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5829
- Target namePXN
- Target descriptionpaxillin
- Target synonymspaxillin, testicular tissue protein Li 134
- HostRabbit
- IsotypeIgG
- Protein IDP49023
- Protein NamePaxillin
- Scientific DescriptionThis gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr31) antibody [HL3129] (GTX640615) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr31) antibody [HL3129] (GTX640615) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640615/GTX640615_45523_20240906_WB_treatment_Pervanadate_24091102_701.webp)
![Paxillin (phospho Tyr31) antibody [HL3129] detects Paxillin (phospho Tyr31) protein by immunofluorescent analysis. Sample: Mock and treated A431 cells were fixed in ice-cold MeOH for 5 min. Green: Paxillin (phospho Tyr31) stained by Paxillin (phospho Tyr31) antibody [HL3129] (GTX640615) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Paxillin (phospho Tyr31) antibody [HL3129] detects Paxillin (phospho Tyr31) protein by immunofluorescent analysis. Sample: Mock and treated A431 cells were fixed in ice-cold MeOH for 5 min. Green: Paxillin (phospho Tyr31) stained by Paxillin (phospho Tyr31) antibody [HL3129] (GTX640615) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX640615/GTX640615_T-45467_20240830_ICC_IF_treatment_pervanadate_24091901_873.webp)






![Untreated (–) and treated (+) HeLa whole cell extract (5 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Paxillin (phospho Tyr118) antibody [HL3384] (GTX641204) diluted at 1:150000 and Paxillin antibody (GTX125891) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641204/GTX641204_T-45572_20241206_WB_treatment_Pervanadate_24121100_507.webp)
![Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Paxillin (phospho Ser273) antibody [HL3588] (GTX641559) diluted at 1:1000 and Paxillin antibody (GTX125891) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641559/GTX641559_T-45628_20241220_WB_treatment_CalyculinA_24122400_949.webp)