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WB analysis of whole cell extracts (20 microg lysate) of A431 (Lane 1) treated for 1 hr with 100 uM of Pervanadate (Lane 2), HeLa (Lane 3) treated for 20 minutes with 50 ng/ml of TNF alpha (lane 4), NIH/3T3 (Lane 5) treated for 10 minutes with 50 ng/ml of PDGF (Lane 6), A549 (Lane 7) treated for 20 minutes with 200 nM of PMA (Lane 8) using GTX24832 Paxillin (phospho Tyr31) antibody. Dilution : 1:500-1:2000
WB analysis of whole cell extracts (20 microg lysate) of A431 (Lane 1) treated for 1 hr with 100 uM of Pervanadate (Lane 2), HeLa (Lane 3) treated for 20 minutes with 50 ng/ml of TNF alpha (lane 4), NIH/3T3 (Lane 5) treated for 10 minutes with 50 ng/ml of PDGF (Lane 6), A549 (Lane 7) treated for 20 minutes with 200 nM of PMA (Lane 8) using GTX24832 Paxillin (phospho Tyr31) antibody. Dilution : 1:500-1:2000
WB analysis of whole cell extracts (20 microg lysate) of A431 (Lane 1) treated for 1 hr with 100 uM of Pervanadate (Lane 2), HeLa (Lane 3) treated for 20 minutes with 50 ng/ml of TNF alpha (lane 4), NIH/3T3 (Lane 5) treated for 10 minutes with 50 ng/ml of PDGF (Lane 6), A549 (Lane 7) treated for 20 minutes with 200 nM of PMA (Lane 8) using GTX24832 Paxillin (phospho Tyr31) antibody. Dilution : 1:500-1:2000

Paxillin (phospho Tyr31) antibody

GTX24832
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
Product group Antibodies
TargetPXN
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Overview

  • Supplier
    GeneTex
  • Product Name
    Paxillin (phospho Tyr31) antibody - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5829
  • Target name
    PXN
  • Target description
    paxillin
  • Target synonyms
    paxillin; testicular tissue protein Li 134
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49023
  • Protein Name
    Paxillin
  • Scientific Description
    This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • The insulin-like growth factor receptor I promotes motility and invasion of bladder cancer cells through Akt- and mitogen-activated protein kinase-dependent activation of paxillin. Metalli D et al., 2010 Jun, Am J Pathol
    Read more

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WB analysis of whole cell extracts (30 microg lysate) of COS-7 (Lane 1) treated with 100 uM Pervanadate for 1 hour (Lane 2), A431 (Lane 3) treated with 100 uM Pervanadate for 1 hour (Lane 4), HeLa (Lane 5) treated with 100 uM Pervanadate for 1 hour (Lane 6) using GTX24833 Paxillin (phospho Tyr118) antibody. Dilution : 1:500
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