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Western Blot Positive WB detected in: Mouse liver tissue, Mouse kidney tissue All lanes: PC antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 130, 58 kDa Observed band size: 130 kDa
Western Blot Positive WB detected in: Mouse liver tissue, Mouse kidney tissue All lanes: PC antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 130, 58 kDa Observed band size: 130 kDa
Western Blot Positive WB detected in: Mouse liver tissue, Mouse kidney tissue All lanes: PC antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 130, 58 kDa Observed band size: 130 kDa

PC Antibody

CSB-PA017511LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPC
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Overview

  • Supplier
    Cusabio
  • Product Name
    PC Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5091
  • Target name
    PC
  • Target description
    pyruvate carboxylase
  • Target synonyms
    PCB; pyruvate carboxylase, mitochondrial; pyruvic carboxylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11498
  • Protein Name
    Pyruvate carboxylase, mitochondrial
  • Scientific Description
    Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Pyruvate Carboxylase/PC using anti-Pyruvate Carboxylase/PC antibody (A03853-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pyruvate Carboxylase/PC antigen affinity purified polyclonal antibody (Catalog # A03853-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pyruvate Carboxylase/PC at approximately 125 kDa. The expected band size for Pyruvate Carboxylase/PC is at 130 kDa.
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