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Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA017514LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA017514LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA017514LA01HU at dilution of 1:100

PCBD1 Antibody

CSB-PA017514LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPCBD1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PCBD1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5092
  • Target name
    PCBD1
  • Target description
    pterin-4 alpha-carbinolamine dehydratase 1
  • Target synonyms
    4-alpha-hydroxy-tetrahydropterin dehydratase; 6-pyruvoyl-tetrahydropterin synthase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1); DCOH; dimerizing cofactor for HNF1; PCBD; PCD; phenylalanine hydroxylase-stimulating protein; PHS; pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha; pterin-4-alpha-carbinolamine dehydratase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61457
  • Protein Name
    Pterin-4-alpha-carbinolamine dehydratase
  • Scientific Description
    Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCBD1 using anti-PCBD1 antibody (A07264-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCBD1 antigen affinity purified polyclonal antibody (Catalog # A07264-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCBD1 at approximately 12 kDa. The expected band size for PCBD1 is at 12 kDa.
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