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PCCA Antibody

LS-C411020
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPCCA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PCCA Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:3000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5095
  • Target name
    PCCA
  • Target description
    propionyl-CoA carboxylase subunit alpha
  • Target synonyms
    propionyl-CoA carboxylase alpha chain, mitochondrial, PCCase alpha subunit, pccA complementation group, propanoyl-CoA:carbon dioxide ligase alpha subunit, propionyl CoA carboxylase, alpha polypeptide, propionyl Coenzyme A carboxylase, alpha polypeptide, propionyl-CoA carboxylase alpha subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCCA using anti-PCCA antibody (A03395-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCCA antigen affinity purified polyclonal antibody (Catalog # A03395-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCCA at approximately 70 kDa. The expected band size for PCCA is at 80 kDa.
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