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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA853490ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA853490ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA853490ESR1HU at dilution of 1:100

PCDH15 Antibody

CSB-PA853490ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPCDH15
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Overview

  • Supplier
    Cusabio
  • Product Name
    PCDH15 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID65217
  • Target name
    PCDH15
  • Target description
    protocadherin related 15
  • Target synonyms
    cadherin-related family member 15; CDHR15; DFNB23; protocadherin-15; USH1F
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96QU1
  • Protein Name
    Protocadherin-15
  • Scientific Description
    Calcium-dependent cell-adhesion protein. Essential for maintenance of normal retinal and cochlear function.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PCDH15 using anti-PCDH15 antibody (A03591-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate, Lane 2: human U2OS whole cell lysate, Lane 3: human Hela whole cell lysate, Lane 4: rat brain tissue lysate, Lane 5: rat lung tissue lysate, Lane 6: mouse brain tissue lysate, Lane 7: mouse lung tissue lysate, Lane 8: mouse kidney tissue lysate, Lane 9: mouse spleen tissue lysate, Lane 10: mouse Neuro-2a whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCDH15 antigen affinity purified polyclonal antibody (Catalog # A03591-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCDH15 at approximately 190KD. The expected band size for PCDH15 is at 216KD.
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