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The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA472895(PCDHA6 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA472895(PCDHA6 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA472895(PCDHA6 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)

PCDHA6 Antibody

CSB-PA472895
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPCDHA6
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Overview

  • Supplier
    Cusabio
  • Product Name
    PCDHA6 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56142
  • Target name
    PCDHA6
  • Target description
    protocadherin alpha 6
  • Target synonyms
    CNR2; CNRN2; CNRS2; CRNR2; KIAA0345-like 8; PCDH-ALPHA6; PCDH-alpha-6; protocadherin alpha-6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UN73
  • Protein Name
    Protocadherin alpha-6
  • Scientific Description
    This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PCDHA6 using anti-PCDHA6 antibody (A13958-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCDHA6 antigen affinity purified polyclonal antibody (Catalog # A13958-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCDHA6 at approximately 150 kDa. The expected band size for PCDHA6 is at 103,120 kDa.
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