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PCDHB14 Antibody (aa166-193)

LS-C163431
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPCDHB14
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PCDHB14 Antibody (aa166-193)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:10 - 1:50), IHC, IHC-P (1:10 - 1:50), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID56122
  • Target name
    PCDHB14
  • Target description
    protocadherin beta 14
  • Target synonyms
    PCDH-BETA14, protocadherin beta-14
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCDHB14 using anti-PCDHB14 antibody (A13272-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCDHB14 antigen affinity purified polyclonal antibody (Catalog # A13272-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCDHB14 at approximately 95 kDa. The expected band size for PCDHB14 is at 88 kDa.
Product group Antibodies
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Anti-PCDHB14 Antibody Picoband(r)A13272-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetPCDHB14
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Product group Antibodies
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Anti-PCDHB14 AntibodyHPA007692
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ReactivityHuman
TargetPCDHB14
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