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Western blot analysis in Jurkat cell line lysates (35ug/lane) using PCDHGC3 antibody. This demonstrates the PCDHGC3 antibody detected the PCDHGC3 protein (arrow).
Western blot analysis in Jurkat cell line lysates (35ug/lane) using PCDHGC3 antibody. This demonstrates the PCDHGC3 antibody detected the PCDHGC3 protein (arrow).
Western blot analysis in Jurkat cell line lysates (35ug/lane) using PCDHGC3 antibody. This demonstrates the PCDHGC3 antibody detected the PCDHGC3 protein (arrow).

PCDHGC3 (Center) Rabbit Polyclonal Antibody

AP53216PU-N
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPCDHGC3
400 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    PCDHGC3 (Center) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5098
  • Target name
    PCDHGC3
  • Target description
    protocadherin gamma subfamily C, 3
  • Target synonyms
    PC43, PCDH-GAMMA-C3, PCDH2, protocadherin gamma-C3, cadherin-like 2, protocadherin 43, protocadherin-2
  • Host
    Rabbit
  • Protein IDQ9UN70
  • Protein Name
    Protocadherin gamma-C3
  • Scientific Description
    PCDHGC3 (Center) rabbit polyclonal antibody, Aff - Purified
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCDHGC3 using anti-PCDHGC3 antibody (A12828-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCDHGC3 antigen affinity purified polyclonal antibody (Catalog # A12828-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCDHGC3 at approximately 118 kDa. The expected band size for PCDHGC3 is at 101,118-137 kDa.
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