pCp-Biotin

Jena Bioscience

pCp-Biotin

5

Research Use Only

pCp-Biotin is recommended for 3-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single Biotin molecule to the 3-OH group of the RNA/miRNA template. The resulting Biotin-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA). Detection of the Biotin moiety can be performed using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Alternatively, Biotin-labeled RNA/miRNA probes can also be purified with Streptavidin-conjugated agarose/magnetic beads.

-20°C

41116133