Western blot using affinity purified anti-Pdcd4 pS457 antibody shows detection of Pdcd4 phosphorylated at Ser 457 (arrowheads). Lanes 1 & 2 each contain 100 ng recombinant Pdcd4. Lanes 3 & 4 each contain 30 ug of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteosome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4). Personal Communication, M Young & A Jansen, NCI, Bethesda, MD.
Western blot using affinity purified anti-Pdcd4 pS457 antibody shows detection of Pdcd4 phosphorylated at Ser 457 (arrowheads). Lanes 1 & 2 each contain 100 ng recombinant Pdcd4. Lanes 3 & 4 each contain 30 ug of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteosome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4). Personal Communication, M Young & A Jansen, NCI, Bethesda, MD.
PDCD4 Rabbit Polyclonal Antibody
TA319401
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat, Xenopus
TargetPDCD4
Overview
- SupplierOriGene
- Product NamePDCD4 Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID27250
- Target namePDCD4
- Target descriptionprogrammed cell death 4
- Target synonymsH731; neoplastic transformation inhibitor protein; nuclear antigen H731; programmed cell death 4 (neoplastic transformation inhibitor); programmed cell death protein 4; protein 197/15a
- HostRabbit
- IsotypeIgG
- Protein IDQ53EL6
- Protein NameProgrammed cell death protein 4
- Scientific DescriptionRabbit polyclonal Pdcd4 phospho S457 antibody (Phospho-specific)
- ReactivityHuman, Mouse, Rat, Xenopus
- Storage Instruction-20°C
- UNSPSC12352203