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ProductsBack/PDCD6IP / ALIX Antibody

PDCD6IP / ALIX Antibody

LS-C331993
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPDCD6IP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PDCD6IP / ALIX Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 30kDa/96kDa, while the observed MW by Western blot was 109kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:200 - 1:2000) The predicted MW is 30kDa/96kDa, while the observed MW by Western blot was 109kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    3.76 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10015
  • Target name
    PDCD6IP
  • Target description
    programmed cell death 6 interacting protein
  • Target synonyms
    AIP1, ALIX, DRIP4, HP95, MCPH29, programmed cell death 6-interacting protein, ALG-2 interacting protein 1, ALG-2-interacting protein X, PDCD6-interacting protein, apoptosis-linked gene 2-interacting protein X, dopamine receptor interacting protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: L02 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, U-87 whole cell lysate, Rat Liver whole cell lysate All lanes: ALIX antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 97, 97, 31 kDa Observed band size: 97 kDa
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Figure 1. Western blot analysis of ALIX using anti-ALIX antibody (PB9770). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALIX antigen affinity purified polyclonal antibody (Catalog # PB9770) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALIX at approximately 96 kDa. The expected band size for ALIX is at 96 kDa.
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