
Inhibition of PDE6C by Sildenafil Citrate (96 reactions).
PDE6C Assay Kit

79501

Product group Assays
Overview
- SupplierBPS Bioscience
- Product NamePDE6C Assay Kit
- Delivery Days Customer7
- CertificationResearch Use Only
- Scientific DescriptionThe PDE6C Assay Kit is a fluorescence polarization (FP), homogeneous, 384-well assay kit designed for the screening and profiling of PDE6C (Phosphodiesterase 6C) inhibitors. This assay takes advantage of a specific fluorescent phosphate-binding nanoparticle. The kit contains enough purified recombinant PDE6C, fluorescent probe, PDE assay buffer, Binding Agent, and diluent for 400 reactions. The assay uses a fluorescein-labeled cyclic guanosine monophosphate (cGMP-FAM for PDE6C), in which the phosphate group is engaged within the cyclic nucleotide. This is a very small molecule that rotates fast (low FP). PDE6C catalyzes the hydrolysis of the phosphodiester bond in the cyclic nucleotide and frees the phosphate group. In a second step the free phosphate group is recognized by a specific phosphate-binding nanobead (Binding Agent) leading to the formation of a large complex, with restricted movement (high FP). FP is proportional to PDE6C activity. This assay requires a fluorescent microplate reader capable of measuring fluorescence polarization (FP) and equipped with the required parts to read the FP signal. For more information FP technology, visit our Tech Note: FP, assay principles and applications. Note: As of July 2024, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.
- Storage InstructionSee Manual
- UNSPSC41116133