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Inhibition of PDE6C by Sildenafil Citrate (96 reactions).
Inhibition of PDE6C by Sildenafil Citrate (96 reactions).
Inhibition of PDE6C by Sildenafil Citrate (96 reactions).

PDE6C Assay Kit

Research Use Only
79501
BPS Bioscience
Product group Assays
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Overview

  • Supplier
    BPS Bioscience
  • Product Name
    PDE6C Assay Kit
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • Scientific Description
    The PDE6C Assay Kit is a fluorescence polarization (FP), homogeneous, 384-well assay kit designed for the screening and profiling of PDE6C (Phosphodiesterase 6C) inhibitors. This assay takes advantage of a specific fluorescent phosphate-binding nanoparticle. The kit contains enough purified recombinant PDE6C, fluorescent probe, PDE assay buffer, Binding Agent, and diluent for 400 reactions. The assay uses a fluorescein-labeled cyclic guanosine monophosphate (cGMP-FAM for PDE6C), in which the phosphate group is engaged within the cyclic nucleotide. This is a very small molecule that rotates fast (low FP). PDE6C catalyzes the hydrolysis of the phosphodiester bond in the cyclic nucleotide and frees the phosphate group. In a second step the free phosphate group is recognized by a specific phosphate-binding nanobead (Binding Agent) leading to the formation of a large complex, with restricted movement (high FP). FP is proportional to PDE6C activity. This assay requires a fluorescent microplate reader capable of measuring fluorescence polarization (FP) and equipped with the required parts to read the FP signal. For more information FP technology, visit our Tech Note: FP, assay principles and applications. Note: As of July 2024, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.
  • Storage Instruction
    See Manual
  • UNSPSC
    41116133