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Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: PDF antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: PDF antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: PDF antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa

PDF Antibody

CSB-PA872538LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPDF
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Overview

  • Supplier
    Cusabio
  • Product Name
    PDF Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64146
  • Target name
    PDF
  • Target description
    peptide deformylase, mitochondrial
  • Target synonyms
    peptide deformylase, mitochondrial; peptide deformylase-like protein; polypeptide deformylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HBH1
  • Protein Name
    Peptide deformylase, mitochondrial
  • Scientific Description
    Removes the formyl group from the N-terminal Met of newly synthesized proteins.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PDF using anti-PDF antibody (A00304-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDF antigen affinity purified polyclonal antibody (Catalog # A00304-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDF at approximately 25 kDa. The expected band size for PDF is at 27,60 kDa.
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