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PDGF-AA Antibody

LS-C17315
LifeSpan BioSciences
ApplicationsFunctional Assay, Western Blot
Product group Antibodies
TargetPDGFA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PDGF-AA Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Western Blot and Growth Inhibition Assays. 2. Prepare a 3T3 cell suspension at 10^4 cells/ml and aliquot 100 ul to each well. 3. Refeed on Day 3. 4. Count wells on Day 5 using an ATP endpoint assay. Wash cell layer once with PBS prior to lysis.. Func, WB (5 - 10 µg/ml) Suitable for use in Western Blot and Growth Inhibition Assays. 2. Prepare a 3T3 cell suspension at 10^4 cells/ml and aliquot 100 ul to each well. 3. Refeed on Day 3. 4. Count wells on Day 5 using an ATP endpoint assay. Wash cell layer once with PBS prior to lysis.
  • Applications
    Functional Assay, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5154
  • Target name
    PDGFA
  • Target description
    platelet derived growth factor subunit A
  • Target synonyms
    PDGF A-chain; PDGF subunit A; PDGF1; PDGF-A; platelet-derived growth factor A-chain; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha polypeptide; platelet-derived growth factor subunit A
  • Host
    Goat
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PDGF AA/PDGFA using anti-PDGF AA/PDGFA antibody (A02257-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGF AA/PDGFA antigen affinity purified polyclonal antibody (Catalog # A02257-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDGF AA/PDGFA at approximately 24 kDa. The expected band size for PDGF AA/PDGFA is at 24 kDa.
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