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Western Blot analysis of COLO205 cells using PDK1 Polyclonal Antibody
Western Blot analysis of COLO205 cells using PDK1 Polyclonal Antibody
Western Blot analysis of COLO205 cells using PDK1 Polyclonal Antibody

PDPK1 Antibody

CSB-PA003730
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDPK1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PDPK1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5170
  • Target name
    PDPK1
  • Target description
    3-phosphoinositide dependent protein kinase 1
  • Target synonyms
    PDK1, PDPK2, PDPK2P, PRO0461, 3-phosphoinositide-dependent protein kinase 1, 3-phosphoinositide-dependent protein kinase 2 pseudogene, PkB kinase like gene 1, Putative 3-phosphoinositide-dependent protein kinase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15530
  • Protein Name
    3-phosphoinositide-dependent protein kinase 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PDPK1 using anti-PDPK1 antibody (PB9735). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate, Lane 2: Rat Lung Tissue Lysate, Lane 3: Mouse Liver Tissue Lysate, Lane 4: Mouse Lung Tissue Lysate, Lane 5: COLO320 Whole Cell Lysate, Lane 6: MCF-7 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDPK1 antigen affinity purified polyclonal antibody (Catalog # PB9735) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDPK1 at approximately 70KD. The expected band size for PDPK1 is at 63KD.
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