Bio-Connect

PDPK1 / PDK1 Antibody (C-Terminus)

LS-C18965
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPDPK1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PDPK1 / PDK1 Antibody (C-Terminus)
  • Delivery Days Customer
    23
  • Application Supplier Note
    This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~60 kD in size corresponding to human PDK-1 protein by western blotting in the appropriate cell lysate or extract. Although untested, this reagent may be useful for immunohistochemistry and immunoprecipitation. Reactivity in other immunoassays is unknown.. ELISA (1:100000), IHC, WB (1:500 - 1:2000) This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~60 kD in size corresponding to human PDK-1 protein by western blotting in the appropriate cell lysate or extract. Although untested, this reagent may be useful for immunohistochemistry and immunoprecipitation. Reactivity in other immunoassays is unknown.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.3 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5170
  • Target name
    PDPK1
  • Target description
    3-phosphoinositide dependent protein kinase 1
  • Target synonyms
    3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; PDK1; PDPK2; PDPK2P; PkB kinase like gene 1; PRO0461
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C or -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetPDPK1
  • SizePrice
Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
TargetPDPK1
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetPDPK1
  • SizePrice
Product group Antibodies
Assay Genie
TargetPDPK1
  • SizePrice
Western Blot analysis of COLO205 cells using PDK1 Polyclonal Antibody
Product group Antibodies
Cusabio
PDPK1 AntibodyCSB-PA003730
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPDPK1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetPDPK1
  • SizePrice
Lane 1: Mouse Liver lysates; Lane 2: Mouse Heart lysates probed with Phospho-PDPK1(Tyr373 + Tyr376) Polyclonal Antibody, Unconjugated (bs-3017R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Product group Antibodies
Bioss Antibodies
ApplicationsWestern Blot, ELISA
TargetPDPK1
  • SizePrice
Product group Antibodies
Abbexa
ApplicationsELISA, ImmunoHistoChemistry
  • SizePrice
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetPDPK1
  • SizePrice
Figure 1. Western blot analysis of PDPK1 using anti-PDPK1 antibody (PB9735). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate, Lane 2: Rat Lung Tissue Lysate, Lane 3: Mouse Liver Tissue Lysate, Lane 4: Mouse Lung Tissue Lysate, Lane 5: COLO320 Whole Cell Lysate, Lane 6: MCF-7 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDPK1 antigen affinity purified polyclonal antibody (Catalog # PB9735) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDPK1 at approximately 70KD. The expected band size for PDPK1 is at 63KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetPDPK1
  • SizePrice