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Western Blot analysis of HuvEc cells using PDXDC1 Polyclonal Antibody
Western Blot analysis of HuvEc cells using PDXDC1 Polyclonal Antibody
Western Blot analysis of HuvEc cells using PDXDC1 Polyclonal Antibody

PDXDC1 Antibody

CSB-PA003734
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDXDC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PDXDC1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23042
  • Target name
    PDXDC1
  • Target description
    pyridoxal dependent decarboxylase domain containing 1
  • Target synonyms
    LP8165; pyridoxal-dependent decarboxylase domain-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6P996
  • Protein Name
    Pyridoxal-dependent decarboxylase domain-containing protein 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PDXDC1 using anti-PDXDC1 antibody (A11375-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDXDC1 antigen affinity purified polyclonal antibody (Catalog # A11375-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDXDC1 at approximately 50 kDa. The expected band size for PDXDC1 is at 87 kDa.
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TargetPDXDC1
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WB analysis of HepG2 and HUVEC cell lysates using GTX87408 PDXDC1 antibody. The lane on the right is blocked with the synthesized peptide.
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Immunofluorescent staining of human cell line HEK 293 shows localization to the Golgi apparatus & vesicles.
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