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PDXK Antibody (YA3496, Mouse)

HY-P83799
MedChem Express
TargetPDXK
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    PDXK Antibody (YA3496, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID8566
  • Target name
    PDXK
  • Target description
    pyridoxal kinase
  • Target synonyms
    C21orf124; C21orf97; epididymis secretory sperm binding protein Li 1a; HEL-S-1a; HMSN6C; PKH; PNK; PRED79; pyridoxal (pyridoxine, vitamin B6) kinase; pyridoxal kinase; pyridoxamine kinase; pyridoxine kinase; vitamin B6 kinase
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    PDXK Antibody (YA3496) is a mouse-derived and non-conjugated IgG1 antibody, targeting to PDXK. It can be applicated for WB, IHC-P, ICC/IF, FC assays, in the background of human, mouse.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PDXK using anti-PDXK antibody (A06683-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDXK antigen affinity purified polyclonal antibody (Catalog # A06683-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDXK at approximately 35 kDa. The expected band size for PDXK is at 35,40 kDa.
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