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Western Blot analysis of A549 cells using PEG3 Polyclonal Antibody
Western Blot analysis of A549 cells using PEG3 Polyclonal Antibody
Western Blot analysis of A549 cells using PEG3 Polyclonal Antibody

PEG3 Antibody

CSB-PA030033
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPEG3
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Overview

  • Supplier
    Cusabio
  • Product Name
    PEG3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5178
  • Target name
    PEG3
  • Target description
    paternally expressed 3
  • Target synonyms
    PW1, ZKSCAN22, ZNF904, ZSCAN24, paternally-expressed gene 3 protein, Kruppel-type zinc finger protein, zinc finger and SCAN domain-containing protein 24
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9GZU2
  • Protein Name
    Paternally-expressed gene 3 protein
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PEG3 using anti-PEG3 antibody (A02541-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEG3 antigen affinity purified polyclonal antibody (Catalog # A02541-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEG3 at approximately 200 kDa. The expected band size for PEG3 is at 181 kDa.
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Anti-PEG3 Antibody Picoband(r)A02541-2-CARRIER-FREE
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WB analysis of A549 cell lysate using GTX34119 PEG3 antibody.
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PEG3 antibodyGTX34119
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TargetPEG3
  • SizePrice
SH-SY5Y cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PEG3 Polyclonal Antibody(bs-1870R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss
PEG3 Polyclonal AntibodyBS-1870R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
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TargetPEG3
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