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Western blot All lanes: PELI1 antibody at 7microg/ml Lane 1: HL60 whole cell lysate Lane 2: Mouse liver tissue Lane 3: THP-1 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa
Western blot All lanes: PELI1 antibody at 7microg/ml Lane 1: HL60 whole cell lysate Lane 2: Mouse liver tissue Lane 3: THP-1 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa
Western blot All lanes: PELI1 antibody at 7microg/ml Lane 1: HL60 whole cell lysate Lane 2: Mouse liver tissue Lane 3: THP-1 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa

PELI1 Antibody

CSB-PA856932ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPELI1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PELI1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57162
  • Target name
    PELI1
  • Target description
    pellino E3 ubiquitin protein ligase 1
  • Target synonyms
    E3 ubiquitin-protein ligase pellino homolog 1, RING-type E3 ubiquitin transferase pellino homolog 1, pellino homolog 1, pellino-1, pellino-related intracellular-signaling molecule, protein pellino homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96FA3
  • Protein Name
    E3 ubiquitin-protein ligase pellino homolog 1
  • Scientific Description
    E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates Lys-63-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1 (1:2000).
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Sample (30 ug of whole cell lysate) A: THP-1 B: HL-60 10% SDS PAGE GTX123102 diluted at 1:500
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