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PELI3 / Pellino 3 Antibody

LS-C498083
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPELI3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PELI3 / Pellino 3 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 35kDa/43kDa/48kDa/50kDa, while the observed MW by Western blot was 50kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 35kDa/43kDa/48kDa/50kDa, while the observed MW by Western blot was 50kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID246330
  • Target name
    PELI3
  • Target description
    pellino E3 ubiquitin protein ligase family member 3
  • Target synonyms
    E3 ubiquitin-protein ligase pellino homolog 3, RING-type E3 ubiquitin transferase pellino homolog 3, pellino homolog 3, protein pellino homolog 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PELI3 using anti-PELI3 antibody (A09622-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PELI3 antigen affinity purified polyclonal antibody (Catalog # A09622-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PELI3 at approximately 51 kDa. The expected band size for PELI3 is at 51 kDa.
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