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WB analysis of various sample lysates using GTX54636 PEPD antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX54636 PEPD antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX54636 PEPD antibody. Dilution : 1:1000 Loading : 25microg per lane

PEPD antibody

GTX54636
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetPEPD
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Overview

  • Supplier
    GeneTex
  • Product Name
    PEPD antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5184
  • Target name
    PEPD
  • Target description
    peptidase D
  • Target synonyms
    aminoacyl-L-proline hydrolase; imidodipeptidase; PROLIDASE; proline dipeptidase; testicular tissue protein Li 138; xaa-Pro dipeptidase; X-Pro dipeptidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12955
  • Protein Name
    Xaa-Pro dipeptidase
  • Scientific Description
    This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PEPD using anti-PEPD antibody (A03417-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat small intestine tissue lysates, Lane 6: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEPD antigen affinity purified polyclonal antibody (Catalog # A03417-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEPD at approximately 60 kDa. The expected band size for PEPD is at 55 kDa.
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