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293T cell lysates probed with PEPP2 Polyclonal Antibody, Unconjugated (bs-12617R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
293T cell lysates probed with PEPP2 Polyclonal Antibody, Unconjugated (bs-12617R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
293T cell lysates probed with PEPP2 Polyclonal Antibody, Unconjugated (bs-12617R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

PEPP2 Polyclonal Antibody

BS-12617R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPLEKHA5
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Overview

  • Supplier
    Bioss
  • Product Name
    PEPP2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID54477
  • Target name
    PLEKHA5
  • Target description
    pleckstrin homology domain containing A5
  • Target synonyms
    PEPP-2, PEPP2, pleckstrin homology domain-containing family A member 5, PH domain-containing family A member 5, phosphoinositol 3-phosphate-binding protein-2, pleckstrin homology domain containing, family A member 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HAU0
  • Protein Name
    Pleckstrin homology domain-containing family A member 5
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PEPP2/PLEKHA5 using anti-PEPP2/PLEKHA5 antibody (A09135-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEPP2/PLEKHA5 antigen affinity purified polyclonal antibody (Catalog # A09135-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEPP2/PLEKHA5 at approximately 130 kDa. The expected band size for PEPP2/PLEKHA5 is at 130 kDa.
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