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PER2 Antibody (C-Terminus)

LS-C17245
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetPER2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PER2 Antibody (C-Terminus)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Western Blot, ELISA, Immunofluorescence and Immunohistochemistry. Western Blot: 1-10 ug/ml using ECL. Immunohistochemistry (paraformaldehyde and frozen): 2-20 ug/ml. Immunofluorescence: 2-20 ug/ml. ELISA: 1:10000-1:100000 using 50-100 ng of control peptide per well.. ELISA (1:10000 - 1:100000), IF (2 - 20 µg/ml), IHC, IHC-Fr, WB (1 - 10 µg/ml) Suitable for use in Western Blot, ELISA, Immunofluorescence and Immunohistochemistry. Western Blot: 1-10 ug/ml using ECL. Immunohistochemistry (paraformaldehyde and frozen): 2-20 ug/ml. Immunofluorescence: 2-20 ug/ml. ELISA: 1:10000-1:100000 using 50-100 ng of control peptide per well.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8864
  • Target name
    PER2
  • Target description
    period circadian regulator 2
  • Target synonyms
    circadian clock protein PERIOD 2; FASPS; FASPS1; hPER2; period 2; period circadian clock 2; period circadian protein 2; period circadian protein homolog 2; period homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PER2 using anti-PER2 antibody (PB9312). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PER2 antigen affinity purified polyclonal antibody (Catalog # PB9312) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PER2 at approximately 130 kDa. The expected band size for PER2 is at 137 kDa.
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IHC image of CSB-PA017787LA01HU diluted at 1:300 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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