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Periplakin (PPL) Polyclonal Antibody

CAU22000
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetPPL
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Overview

  • Supplier
    Biomatik
  • Product Name
    Periplakin (PPL) Polyclonal Antibod
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5493
  • Target name
    PPL
  • Target description
    periplakin
  • Target synonyms
    190 kDa paraneoplastic pemphigus antigen; 195 kDa cornified envelope precursor protein; periplakin
  • Host
    Rabbit
  • Protein IDO60437
  • Protein Name
    Periplakin
  • Scientific Description
    The Periplakin (PPL) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Periplakin using anti-Periplakin antibody (A02098-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human SK-OV-3 whole cell lysates, Lane 6: human PANC-1 whole cell lysates, Lane 7: human SGC-7801 whole cell lysates, Lane 8: rat lung tissue lysates, Lane 9: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Periplakin antigen affinity purified polyclonal antibody (Catalog # A02098-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Periplakin at approximately 202 kDa. The expected band size for Periplakin is at 205 kDa.
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