
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-PERK Polyclonal Antibody, Unconjugated (bs-2469R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
PERK Polyclonal Antibody
BS-2469R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetEIF2AK3
Overview
- SupplierBioss
- Product NamePERK Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID9451
- Target nameEIF2AK3
- Target descriptioneukaryotic translation initiation factor 2 alpha kinase 3
- Target synonymsPEK, PERK, WRS, eukaryotic translation initiation factor 2-alpha kinase 3, PKR-like ER kinase, PRKR-like endoplasmic reticulum kinase, pancreatic EIF2-alpha kinase, pancreatic eIF-2alpha kinase, protein tyrosine kinase EIF2AK3
- HostRabbit
- IsotypeIgG
- Protein IDQ9NZJ5
- Protein NameEukaryotic translation initiation factor 2-alpha kinase 3
- Storage Instruction-20°C
- UNSPSC12352203
References
- Cui J, Liu Y, Chang X, et al. Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress- and Ca(2+) Imbalance-Mediated Endoplasmic Reticulum Stress. Oxid Med Cell Longev. 2019,2019:2593742. doi: 10.1155/2019/2593742Read this paper
- Wang L, Li X. Radioprotective effect of Hohenbuehelia serotina polysaccharides through mediation of ER apoptosis pathway in vivo. Int J Biol Macromol. 2019,127:18-26. doi: 10.1016/j.ijbiomac.2018.12.267Read this paper
- Li H, Wang B, Zhang H, et al. Apoptosis Induction by Iron Radiation via Inhibition of Autophagy in Trp53(+/-) Mouse Testes: Is Chronic Restraint-Induced Stress a Modifying Factor? Int J Biol Sci. 2018,14(9):1109-1121. doi: 10.7150/ijbs.22805Read this paper
- Duan J, Kang J, Deng T, et al. Exposure to DBP and High Iodine Aggravates Autoimmune Thyroid Disease Through Increasing the Levels of IL-17 and Thyroid-Binding Globulin in Wistar Rats. Toxicol Sci. 2018,163(1):196-205. doi: 10.1093/toxsci/kfy019Read this paper
- Li L, Cui J, Liu Z, et al. Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function. Toxicol Lett. 2018,285:156-167. doi: 10.1016/j.toxlet.2018.01.004Read this paper
- Yu H, Zhang H, Zhao W, et al. Gypenoside Protects against Myocardial Ischemia-Reperfusion Injury by Inhibiting Cardiomyocytes Apoptosis via Inhibition of CHOP Pathway and Activation of PI3K/Akt Pathway In Vivo and In Vitro. Cell Physiol Biochem. 2016,39(1):123-36. doi: 10.1159/000445611Read this paper









