
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-PERK (Thr980) Polyclonal Antibody, Unconjugated (bs-3330R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
PERK(Thr980) Polyclonal Antibody
BS-3330R

ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Rabbit, Rat
TargetEif2ak3
Overview
- SupplierBioss Antibodies
- Product NamePERK(Thr980) Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID13666
- Target nameEif2ak3
- Target descriptioneukaryotic translation initiation factor 2 alpha kinase 3
- Target synonymseukaryotic translation initiation factor 2-alpha kinase 3; pancreatic eIF2-alpha kinase; PE; Pek; Perk; PRKR-like endoplasmic reticulum kinase
- HostRabbit
- IsotypeIgG
- Protein IDQ9Z2B5
- Protein NameEukaryotic translation initiation factor 2-alpha kinase 3
- ReactivityBovine, Canine, Human, Mouse, Porcine, Rabbit, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
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- Functional Characterization of a Novel Truncating Mutation in Lamin A/C Gene in a Family with a Severe Cardiomyopathy with Conduction Defects. Gerbino A et al., 2017, Cell Physiol BiochemRead more
- Tetramethylpyrazine suppresses angiotensin II-induced soluble epoxide hydrolase expression in coronary endothelium via anti-ER stress mechanism. Mak SK et al., 2017 Dec 1, Toxicol Appl PharmacolRead more