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ProductsBack/PF4 antibody [14M15]

PF4 antibody [14M15]

GTX53301
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse
TargetPf4
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Overview

  • Supplier
    GeneTex
  • Product Name
    PF4 antibody [14M15]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:250 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    14M15
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID56744
  • Target name
    Pf4
  • Target description
    platelet factor 4
  • Target synonyms
    Cxcl4, Scyb4, platelet factor 4, C-X-C motif chemokine 4, chemokine (C-X-C motif) ligand 4
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDQ9Z126
  • Protein Name
    Platelet factor 4
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PF4 using anti-PF4 antibody (PB10012). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody (Catalog # PB10012) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PF4 at approximately 11 kDa. The expected band size for PF4 is at 11 kDa.
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