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Western Blot analysis of K562 cells using PFKM Polyclonal Antibody
Western Blot analysis of K562 cells using PFKM Polyclonal Antibody
Western Blot analysis of K562 cells using PFKM Polyclonal Antibody

PFKM Antibody

CSB-PA003744
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPFKM
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Overview

  • Supplier
    Cusabio
  • Product Name
    PFKM Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5213
  • Target name
    PFKM
  • Target description
    phosphofructokinase, muscle
  • Target synonyms
    6-phosphofructo-1-kinase; 6-phosphofructokinase type A; 6-phosphofructokinase, muscle type; ATP-dependent 6-phosphofructokinase, muscle type; ATP-PFK; GSD7; PFK1; PFK-1; PFKA; PFK-A; PFKX; phosphofructo-1-kinase isozyme A; phosphofructokinase 1; phosphofructokinase, polypeptide X; phosphofructokinase-M; phosphohexokinase; PPP1R122; protein phosphatase 1, regulatory subunit 122
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08237
  • Protein Name
    ATP-dependent 6-phosphofructokinase, muscle type
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PFKM using anti-PFKM antibody (A00437-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFKM antigen affinity purified polyclonal antibody (Catalog # A00437-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PFKM at approximately 85 kDa. The expected band size for PFKM is at 85 kDa.
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