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ProductsBack/PFKP Antibody - C-terminal region (OAAB17503)

PFKP Antibody - C-terminal region (OAAB17503)

OAAB17503
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetPFKP
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    PFKP Antibody - C-terminal region (OAAB17503)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Gene ID5214
  • Target name
    PFKP
  • Target description
    phosphofructokinase, platelet
  • Target synonyms
    6-phosphofructokinase type C; 6-phosphofructokinase, platelet type; ATP-dependent 6-phosphofructokinase, platelet type; ATP-PFK; PFK-C; PFKF; PFK-P; phosphofructo-1-kinase isozyme C; phosphofructokinase 1; phosphohexokinase
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PFKP using anti-PFKP antibody (A07337-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFKP antigen affinity purified polyclonal antibody (Catalog # A07337-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PFKP at approximately 86 kDa. The expected band size for PFKP is at 86, 80-86 kDa.
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