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Western Blot analysis of HuvEc cells using PFK-C Polyclonal Antibody
Western Blot analysis of HuvEc cells using PFK-C Polyclonal Antibody
Western Blot analysis of HuvEc cells using PFK-C Polyclonal Antibody

PFKP Antibody

CSB-PA003749
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPFKP
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Overview

  • Supplier
    Cusabio
  • Product Name
    PFKP Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5214
  • Target name
    PFKP
  • Target description
    phosphofructokinase, platelet
  • Target synonyms
    6-phosphofructokinase type C; 6-phosphofructokinase, platelet type; ATP-dependent 6-phosphofructokinase, platelet type; ATP-PFK; PFK-C; PFKF; PFK-P; phosphofructo-1-kinase isozyme C; phosphofructokinase 1; phosphohexokinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01813
  • Protein Name
    ATP-dependent 6-phosphofructokinase, platelet type
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PFKP using anti-PFKP antibody (A07337-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFKP antigen affinity purified polyclonal antibody (Catalog # A07337-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PFKP at approximately 86 kDa. The expected band size for PFKP is at 86, 80-86 kDa.
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Lane 1: Hela cell lysates; Lane 2: Jurkat cell lysates; Lane 3: COS-7 cell lysates; Lane 4: Mouse heart tissue lysates probed with PFKP Monoclonal Antibody, Unconjugated (bsm-51630M) at 1:4000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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