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Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A549 cells, A375 cells, Primary antibody: CSB-PA230596(PGBD4 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A549 cells, A375 cells, Primary antibody: CSB-PA230596(PGBD4 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A549 cells, A375 cells, Primary antibody: CSB-PA230596(PGBD4 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds

PGBD4 Antibody

CSB-PA230596
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPGBD4
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Overview

  • Supplier
    Cusabio
  • Product Name
    PGBD4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID161779
  • Target name
    PGBD4
  • Target description
    piggyBac transposable element derived 4
  • Target synonyms
    piggyBac transposable element-derived protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96DM1
  • Protein Name
    PiggyBac transposable element-derived protein 4
  • Scientific Description
    The piggyBac family of proteins, found in diverse animals, are transposases related to the transposase of the canonical piggyBac transposon from the moth, Trichoplusia ni. This family also includes genes in several genomes, including human, that appear to have been derived from the piggyBac transposons. This gene belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel, with no obvious relationship to other transposases, or other known protein families.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PGBD4 using anti-PGBD4 antibody (A19178-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGBD4 antigen affinity purified polyclonal antibody (Catalog # A19178-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGBD4 at approximately 70 kDa. The expected band size for PGBD4 is at 67 kDa.
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