HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PTGES2 Antibody(bs-2639R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PTGES2 Antibody(bs-2639R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
PTGES2 Polyclonal Antibody
BS-2639R
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Human, Mouse, Porcine, Rat
TargetPtges2
Overview
- SupplierBioss
- Product NamePTGES2 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID96979
- Target namePtges2
- Target descriptionprostaglandin E synthase 2
- Target synonyms0610038H10Rik, Gbf1, Mpges2, Pges2, prostaglandin E synthase 2, GATE-binding factor 1, mPGES-2, microsomal prostaglandin E synthase 2
- HostRabbit
- IsotypeIgG
- Protein IDQ8BWM0
- Protein NameProstaglandin E synthase 2
- ReactivityBovine, Canine, Chicken, Human, Mouse, Porcine, Rat
- Storage Instruction-20°C
- UNSPSC41116161
References
- Activated hepatic stellate cells directly induce pathogenic Th17 cells in chronic hepatitis B virus infection. Liu Y et al., 2017 Oct 1, Exp Cell ResRead this paper
- The Antinociceptive Effect of Light-Emitting Diode Irradiation on Incised Wounds Is Correlated with Changes in Cyclooxygenase 2 Activity, Prostaglandin E2, and Proinflammatory Cytokines. Chia YY et al., 2017, Pain Res ManagRead this paper
Datasheet
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Product group Antibodies
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse
TargetPtges2
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