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Western Blot analysis of HepG2, 293T, hela and NIH/3T3 cell using PGK2 Polyclonal Antibody at dilution of 1:600
Western Blot analysis of HepG2, 293T, hela and NIH/3T3 cell using PGK2 Polyclonal Antibody at dilution of 1:600
Western Blot analysis of HepG2, 293T, hela and NIH/3T3 cell using PGK2 Polyclonal Antibody at dilution of 1:600

PGK2 Polyclonal Antibody

E-AB-10901
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    PGK2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PGK2 using anti-PGK2 antibody (A08946-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGK2 antigen affinity purified polyclonal antibody (Catalog # A08946-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGK2 at approximately 45 kDa. The expected band size for PGK2 is at 45 kDa.
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