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Western Blot analysis of COLO205 cells using PGLS Polyclonal Antibody
Western Blot analysis of COLO205 cells using PGLS Polyclonal Antibody
Western Blot analysis of COLO205 cells using PGLS Polyclonal Antibody

PGLS Antibody

CSB-PA003752
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPGLS
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Overview

  • Supplier
    Cusabio
  • Product Name
    PGLS Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25796
  • Target name
    PGLS
  • Target description
    6-phosphogluconolactonase
  • Target synonyms
    6PGL, HEL-S-304, 6-phosphogluconolactonase, epididymis secretory protein Li 304, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95336
  • Protein Name
    6-phosphogluconolactonase
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PGLS using anti-PGLS antibody (A02553-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human CCRF-CEM whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGLS antigen affinity purified polyclonal antibody (Catalog # A02553-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGLS at approximately 28 kDa. The expected band size for PGLS is at 28 kDa.
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