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ProductsBack/PGM2 Rabbit Polyclonal Antibody
WB Suggested Anti-PGM2 Antibody Titration: 1.0 ug/ml Positive Control: THP-1 Whole Cell
WB Suggested Anti-PGM2 Antibody Titration: 1.0 ug/ml Positive Control: THP-1 Whole Cell
WB Suggested Anti-PGM2 Antibody Titration: 1.0 ug/ml Positive Control: THP-1 Whole Cell

PGM2 Rabbit Polyclonal Antibody

TA358508
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetPGM2
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    PGM2 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID55276
  • Target name
    PGM2
  • Target description
    phosphoglucomutase 2
  • Target synonyms
    glucose phosphomutase 2; MSTP006; PGM 2; phosphodeoxyribomutase; phosphoglucomutase-2
  • Host
    Rabbit
  • Protein IDQ96G03
  • Protein Name
    Phosphoglucomutase-2
  • Scientific Description
    PGM2 Antibody - C-terminal region
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of PGM2 using anti-PGM2 antibody (A07690-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGM2 antigen affinity purified polyclonal antibody (Catalog # A07690-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGM2 at approximately 75 kDa. The expected band size for PGM2 is at 68 kDa.
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