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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA017873LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA017873LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA017873LA01HU at dilution of 1:100

PGP Antibody

CSB-PA017873LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPGP
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Overview

  • Supplier
    Cusabio
  • Product Name
    PGP Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID283871
  • Target name
    PGP
  • Target description
    phosphoglycolate phosphatase
  • Target synonyms
    AUM, G3PP, PGPase, glycerol-3-phosphate phosphatase, aspartate-based ubiquitous Mg(2+)-dependent phosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDA6NDG6
  • Protein Name
    Glycerol-3-phosphate phosphatase
  • Scientific Description
    Glycerol-3-phosphate phosphatase hydrolyzing glycerol-3-phosphate into glycerol. Thereby, regulates the cellular levels of glycerol-3-phosphate a metabolic intermediate of glucose, lipid and energy metabolism. Was also shown to have a 2-phosphoglycolate phosphatase activity and a tyrosine-protein phosphatase activity. However, their physiological relevance is unclear (PubMed:26755581). In vitro, has also a phosphatase activity toward ADP, ATP, GDP and GTP (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PGP using anti-PGP antibody (A00932-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGP antigen affinity purified polyclonal antibody (Catalog # A00932-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGP at approximately 36 kDa. The expected band size for PGP is at 34 kDa.
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