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ProductsBack/PGRMC1 / MPR Antibody (aa145-195)

PGRMC1 / MPR Antibody (aa145-195)

LS-C762080
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPGRMC1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PGRMC1 / MPR Antibody (aa145-195)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunoprecipitation Range: 2-10 microg/mg lysate. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:2000 - 1:10000), IP, WB (1:1000 - 1:5000) Immunoprecipitation Range: 2-10 µg/mg lysate. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10857
  • Target name
    PGRMC1
  • Target description
    progesterone receptor membrane component 1
  • Target synonyms
    Dap1, HPR6.6, IZA, MPR, membrane-associated progesterone receptor component 1, progesterone binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of PGRMC1 using anti-PGRMC1 antibody (PB9775). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Kidney Tissue Lysate at 50ug, Lane 3: Mouse Liver Tissue Lysate at 50ug, Lane 4: Mouse Kidney Tissue Lysate at 50ug, Lane 5: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGRMC1 antigen affinity purified polyclonal antibody (Catalog # PB9775) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGRMC1 at approximately 26 kDa. The expected band size for PGRMC1 is at 26 kDa.
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