WB analysis of various samples using GTX03254 PHD1 antibody [GT1342]. Dilution : 1:1000 Loading : 25microg per lane
![Various whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with PHD1 antibody [GT1342] (GTX03254) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX03254/GTX03254_4000000230_20210625_WB_2_w_23053123_378.webp "Various whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with PHD1 antibody [GT1342] (GTX03254) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 microg) was separated by 10% SDS-PAGE, and the membrane was blotted with PHD1 antibody [GT1342] (GTX03254) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX03254/GTX03254_4000000230_20210625_WB_w_23053123_755.webp "Whole cell extract (30 microg) was separated by 10% SDS-PAGE, and the membrane was blotted with PHD1 antibody [GT1342] (GTX03254) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
WB analysis of various samples using GTX03254 PHD1 antibody [GT1342]. Dilution : 1:1000 Loading : 25microg per lane
PHD1 antibody [GT1342]
GTX03254
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEGLN2
Overview
- SupplierGeneTex
- Product NamePHD1 antibody [GT1342]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1342
- Concentration0.18 mg/ml
- ConjugateUnconjugated
- Gene ID112398
- Target nameEGLN2
- Target descriptionegl-9 family hypoxia inducible factor 2
- Target synonymsegl nine homolog 2; EIT6; EIT-6; estrogen-induced tag 6; HIFPH1; HIF-PH1; HIF-prolyl hydroxylase 1; HPH-1; HPH-3; hypoxia-inducible factor prolyl hydroxylase 1; PHD1; prolyl hydroxylase domain-containing protein 1; prolyl hydroxylase EGLN2
- HostRabbit
- IsotypeIgG
- Protein IDQ96KS0
- Protein NameEgl nine homolog 2
- Scientific DescriptionThe hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. [provided by RefSeq, Feb 2011]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203