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Western Blot analysis of HeLa cells using PHF1 Polyclonal Antibody
Western Blot analysis of HeLa cells using PHF1 Polyclonal Antibody
Western Blot analysis of HeLa cells using PHF1 Polyclonal Antibody

PHF1 Antibody

CSB-PA003754
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPHF1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PHF1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5252
  • Target name
    PHF1
  • Target description
    PHD finger protein 1
  • Target synonyms
    MTF2L2, PCL1, PHF2, TDRD19C, hPHF1, PHD finger protein 1, hPCl1, polycomb-like 1, polycomb-like protein 1, testicular tissue protein Li 140, tudor domain containing 19C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43189
  • Protein Name
    PHD finger protein 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PHF1 using anti-PHF1 antibody (A03758-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates, Lane 3: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF1 antigen affinity purified polyclonal antibody (Catalog # A03758-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHF1 at approximately 62 kDa. The expected band size for PHF1 is at 62 kDa.
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