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WB analysis of various sample lysates using GTX65536 PHF11 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX65536 PHF11 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX65536 PHF11 antibody. Dilution : 1:1000 Loading : 25microg per lane

PHF11 antibody

GTX65536
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetPHF11
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Overview

  • Supplier
    GeneTex
  • Product Name
    PHF11 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51131
  • Target name
    PHF11
  • Target description
    PHD finger protein 11
  • Target synonyms
    APY, BCAP, IGEL, IGER, IGHER, NY-REN-34, NYREN34, PHD finger protein 11, BRCA1 C-terminus-associated protein, IgE responsiveness (atopic), renal carcinoma antigen NY-REN-34
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UIL8
  • Protein Name
    PHD finger protein 11
  • Scientific Description
    This gene encodes a protein containing a PHD (plant homeodomain) type zinc finger. This gene has been identified in some studies as a candidate gene for asthma. Naturally-occurring readthrough transcription may occur from the upstream SETDB2 (SET domain bifurcated 2) gene to this locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PHF11 using anti-PHF11 antibody (A09621-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF11 antigen affinity purified polyclonal antibody (Catalog # A09621-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHF11 at approximately 34 kDa. The expected band size for PHF11 is at 38,34 kDa.
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