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Western Blot Positive WB detected in: Mouse liver tissue, Mouse kidney tissue All lanes: PHF21A antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 75, 71 kDa Observed band size: 75 kDa
Western Blot Positive WB detected in: Mouse liver tissue, Mouse kidney tissue All lanes: PHF21A antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 75, 71 kDa Observed band size: 75 kDa
Western Blot Positive WB detected in: Mouse liver tissue, Mouse kidney tissue All lanes: PHF21A antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 75, 71 kDa Observed band size: 75 kDa

PHF21A Antibody

CSB-PA017911LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPHF21A
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Overview

  • Supplier
    Cusabio
  • Product Name
    PHF21A Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51317
  • Target name
    PHF21A
  • Target description
    PHD finger protein 21A
  • Target synonyms
    BHC80; BHC80a; BM-006; BRAF35/HDAC2 complex (80 kDa); BRAF35-HDAC complex protein BHC80; IDDBCS; NEDMS; PHD finger protein 21A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96BD5
  • Protein Name
    PHD finger protein 21A
  • Scientific Description
    Component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it may act as a scaffold. Inhibits KDM1A-mediated demethylation of Lys-4 of histone H3 in vitro, suggesting a role in demethylation regulation.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PHF21A using anti-PHF21A antibody (A08507-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates Lane 2: rat brain tissue lysates Lane 3: mouse brain tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF21A antigen affinity purified polyclonal antibody (Catalog # A08507-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHF21A at approximately 75-95KD. The expected band size for PHF21A is at 75KD.
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