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Western Blot analysis of HepG2 cells using PHKG1 Polyclonal Antibody
Western Blot analysis of HepG2 cells using PHKG1 Polyclonal Antibody
Western Blot analysis of HepG2 cells using PHKG1 Polyclonal Antibody

PHKG1 Antibody

CSB-PA003756
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPHKG1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PHKG1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5260
  • Target name
    PHKG1
  • Target description
    phosphorylase kinase catalytic subunit gamma 1
  • Target synonyms
    PHKG, phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform, PHK-gamma-M, phosphorylase kinase gamma subunit 1, phosphorylase kinase subunit gamma-1, phosphorylase kinase, gamma 1 (muscle), serine/threonine-protein kinase PHKG1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16816
  • Protein Name
    Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PHKG1 using anti-PHKG1 antibody (A12257-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHKG1 antigen affinity purified polyclonal antibody (Catalog # A12257-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHKG1 at approximately 40 kDa. The expected band size for PHKG1 is at 45 kDa.
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