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PHKG1 Antibody (N-Terminus)

LS-C170257
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPHKG1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PHKG1 Antibody (N-Terminus)
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5260
  • Target name
    PHKG1
  • Target description
    phosphorylase kinase catalytic subunit gamma 1
  • Target synonyms
    PHKG; PHK-gamma-M; phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform; phosphorylase kinase gamma subunit 1; phosphorylase kinase subunit gamma-1; phosphorylase kinase, gamma 1 (muscle); serine/threonine-protein kinase PHKG1
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PHKG1 using anti-PHKG1 antibody (A12257-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHKG1 antigen affinity purified polyclonal antibody (Catalog # A12257-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHKG1 at approximately 40 kDa. The expected band size for PHKG1 is at 45 kDa.
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