ICC/IF analysis of HeLa cells using GTX00700 PHLDA3 antibody [4B6].
Dilution : 1:1000
Fixation : 4% PFA
Permeabilization: 0.25% Triton X-100 in PBS for 10 min
![WB analysis of MDA-MB-468 cell lysate using GTX00700 PHLDA3 antibody [4B6]. PHLDA3 protein was induced by Ad-p53 in MDA-MB-468 cells (left). PHLDA3 expression was inhibited by shRNA targeting PHLDA3 (right).](https://www.genetex.com/upload/website/prouct_img/normal/GTX00700/GTX00700_20191104_WB_w_23053121_717.webp "WB analysis of MDA-MB-468 cell lysate using GTX00700 PHLDA3 antibody [4B6]. PHLDA3 protein was induced by Ad-p53 in MDA-MB-468 cells (left). PHLDA3 expression was inhibited by shRNA targeting PHLDA3 (right).")
ICC/IF analysis of HeLa cells using GTX00700 PHLDA3 antibody [4B6].
Dilution : 1:1000
Fixation : 4% PFA
Permeabilization: 0.25% Triton X-100 in PBS for 10 min
PHLDA3 antibody [4B6]
GTX00700
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPHLDA3
Overview
- SupplierGeneTex
- Product NamePHLDA3 antibody [4B6]
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4B6
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID23612
- Target namePHLDA3
- Target descriptionpleckstrin homology like domain family A member 3
- Target synonymsTIH1, pleckstrin homology-like domain family A member 3, TDAG51/Ipl homolog 1, pleckstrin homology-like domain, family A, member 2
- HostMouse
- IsotypeIgG2b
- Protein IDQ9Y5J5
- Protein NamePleckstrin homology-like domain family A member 3
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Kawase T, Ohki R, Shibata T, et al. PH domain-only protein PHLDA3 is a p53-regulated repressor of Akt. Cell. 2009,136(3):535-50. doi: 10.1016/j.cell.2008.12.002Read this paper
Datasheet
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